Cryopreservation of Cells and Tissues

Thursday August 29, 2019 from 08:30 to 10:10

Room: 519b

TS-404.2 Instant cryopreservation of porcine heart valves and aortic segments in low-viscous polydimethylsiloxane - first results

Stanislav Lauk-Dubitskiy, Russian Federation

Junior research associate
"Cryology" center
The Bauman Moscow State Technical University

Abstract

Instant cryopreservation of porcine heart valves and aortic segments in low-viscous polydimethylsiloxane - first results.

Stanislav Lauk-Dubitskiy1, Lyubov Severgina2, Igor Korovin3, Alexey Shakurov1, Alexander Pushkarev1, Roman Komarov4, Dmitrii Britikov5, Ivan Novikov6.

1The Bauman Moscow State Technical University, Moscow, Russian Federation; 2Anatomic pathology department, I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation; 3Medical school “Medicine of the future”, I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation; 4Clinic of cardiovascular surgery, I.M. Sechenov First Moscow State Medical University, Moscow, Russian Federation; 5A.N. Bakulev National Medical Research Center of Cardiovascular Surgery, Moscow, Russian Federation; 6FGBU «NII Eyes disease», Moscow, Russian Federation

 

Aim. To evaluate the safety of heart valves cryopreservation in low-viscous polydimethylsiloxane (PDMS).

Materials and methods.  15 porcine heart valves (mitral and aortic / 1.0 cm) and aortic segments (2.0 cm) were cryopreserved in PDMS (1cSt, -75°C, 1L, temperature control) with stirring (150 turns per minute) for 40 sec, and then thawed in the PDMS (5cSt, +24°C, 1L, 1 min). The cryopreservation protocol was applied with speed of freezing and thawing close to 200°C/min without cryoprotectants and LN2. The quality of cryopreservation was assessed by histological methods (Orcein, Van Gieson, H&E).

Results. There were no critical differences between the valves and aortic segments of experimental and control groups. 

Conclusion. Using of PDMS is feasible for instant cryopreservation of vascular tissue for their implantation as homografts or biobanking with possible additional usage of the inner hydrophilic covering of tissue to prevent hydrophobization.

© 2021 ICR 2019